Dear Core User,

Due to the current situation with COVID-19, per directives from Senior management at Mass General Brigham and the Hospitals, this research core facilities will have a hiatus in their service offering. We will not be offering service till we are advised to resume operation.

Please stay safe in this challenging time and we look forward to working with you in the future.

Flow Cytometry Core

To provide high standard cell sorting and analysis services to the research community at the Brigham and Women’s Hospital and the Longwood Medical area.

Personnel/Contact Information:

Director:
Director: Ronglih Liao, Ph.D.


Manager, BWH flow cytometry core:

Grigoriy Losyev - Manager, BWH flow cytometry core

617-525-4748

GLOSYEV@PARTNERS.ORG


Administrator, BWH flow cytometry core:
Rick Bowman

617-525-4858

fbowman@bwh.harvard.edu


Location of Core: 
77 Avenue Louis Pasteur, NRB 435E, Boston


Major Equipment: 

https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-cell-sorters/facsaria-fusion


BD Accuri C6 4-color Analyzer
Cytek DxP12 10-color Analyzer


Getting Started:

For more information on the core, please visit the website here


Online sign-up:
Please register first with Grigoriy Losyev at GLOSYEV@PARTNERS.ORG

Sample Preparation:

What samples should I bring?

  • All users must always bring single stained compensation samples each stained with one color AND one unstained tube. Cell suspensions should be passed through a 35um nylon mesh filter (BD Biosciences part # 352235) right before sorting.
  • Don’t have enough cells for compensation?User can purchase compensation beads from BD BD CompBeads 552845 ‘Anti-Rat/Hamster’ for mouse samples BD CompBeads 552843 ‘Anti-Mouse’ for almost all human samples.