Dear Core User,
Due to the current situation with COVID-19, per directives from Senior management at Mass General Brigham and the Hospitals, this research core facilities will have a hiatus in their service offering. We will not be offering service till we are advised to resume operation.
Please stay safe in this challenging time and we look forward to working with you in the future.
**We will be operational until Friday March 20 at 5:00 and will then close operations in accordance with Partners/BWH guidelines. We expect to be closed for at least 6 weeks. Thank you for your continued use of our core and please stay safe.**
Seahorse Core
The Seahorse Core supports the use of an XFe24 Extracellular Flux Analyzer from Seahorse Biosciences to perform sensitive measurements of oxygen consumption rate and extracellular acidification rate in cultured cells, tissues, or model organisms in a 24-well format.
Contact Information
Director: William Oldham, M.D., Ph.D.
Phone: 617-525-4834
Email: woldham@bwh.harvard.edu
Administrator: Rick Bowman
Phone: 617-525-4858
Email: fbowman@bwh.harvard.edu
Core email:
Facilites and Equipment
Location: Harvard New Research Building 631, 77 Avenue Louis Pasteur, Boston
Hours: M-F, 8-5; Two assays run per day (morning and afternoon).
Equipment: XFe24 Extracellular Flux Analyzer, Prep Station
Services
- Basic Assay – core provides assay plate and assay cartridge – user provides samples in the assay plate, assay medium, and injection reagents
- Glyco Stress Assay – core provides assay plate, assay cartridge, Seahorse medium with supplements, and Seahorse reagents – user provides samples in the assay plate
- Mito Stress Assay – core provides assay plate, assay cartridge, Seahorse medium with supplements, and reagents – user provides samples in the assay plate
All services include use of the Seahorse Prep Station liquid handling system and non-CO2 incubator, cartridge hydration on the day prior to the assay, and technical assistance. Users are responsible for preparing media and injection reagents, performing media exchanges, loading the assay cartridges, running the assay, and analyzing the data. All new users require training.
User Fees
Our new fees as of 1/1/2020 will be:
| Services | PHS | Academic | Industry |
|---|---|---|---|
| Seahorse Test without Suplies (Basic Assay) | $395 | $568.80 | $628.05 |
| Glyco Stress Assay with Supplies | $565 | $813.60 | $898.35 |
| Mito Stress Assay with Supplies | $505 | $727.20 | $802.95 |
| Cartridge | $80 | $115.20 | $127.20 |
| Training | $70/hr | $100.80/hr | $111.30/hr |
| Services | PHS | Academic | Industry |
|---|---|---|---|
| Basic Assay | $375 | $540 | $710 |
| Glyco Stress Assay | $540 | $777 | $1020 |
| Mito Stress Assay | $482 | $695 | $910 |
| Training | $65/h | $94/h | $130/h |
Getting Started
Navigate to https://researchcores.partners.org/ and create a user account based on the instructions in the quick guide (see link on the Research Cores Home Page). Provide the fund information that will be used to pay for services.
